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Arthur T Knackerbracket has found the following story:

By adding infrared capability to the ubiquitous, standard optical microscope, researchers at the University of Illinois at Urbana-Champaign hope to bring cancer diagnosis into the digital era.

Pairing infrared measurements with high-resolution optical images and machine learning algorithms, the researchers created digital biopsies that closely correlated with traditional pathology techniques and also outperformed state-of-the-art infrared microscopes.

Led by Rohit Bhargava, a professor of bioengineering and the director of the Cancer Center at Illinois, the group published its results in the Proceedings of the National Academy of Sciences.

"The advantage is that no stains are required, and both the organization of cells and their chemistry can be measured. Measuring the chemistry of tumor cells and their microenvironment can lead to better cancer diagnoses and better understanding of the disease," Bhargava said.

[...] Bhargava's group developed its hybrid microscope by adding an infrared laser and a specialized microscope lens, called an interference objective, to an optical camera. The infrared-optical hybrid measures both infrared data and a high-resolution optical image with a light microscope -- the kind ubiquitous in clinics and labs.

"We built the hybrid microscope from off-the-shelf components. This is important because it allows others to easily build their own microscope or upgrade an existing microscope," said Martin Schnell, a postdoctoral fellow in Bhargava's group and first author of the paper.

Combining the two techniques harnesses the strengths of both, the researchers said. It has the high resolution, large field-of-view and accessibility of an optical microscope. Furthermore, infrared data can be analyzed computationally, without adding any dyes or stains that can damage tissues. Software can recreate different stains or even overlap them to create a more complete, all-digital picture of what's in the tissue.

The researchers verified their microscope by imaging breast tissue samples, both healthy and cancerous, and comparing the results of the hybrid microscope's computed "dyes" with those from the traditional staining technique. The digital biopsy closely correlated with the traditional one.

Furthermore, the researchers found that their infrared-optical hybrid outperformed state-of-the-art in infrared microscopes in several ways: It has 10 times larger coverage, greater consistency and four times higher resolution, allowing infrared imaging of larger samples, in less time, with unprecedented detail.

Journal Reference:

Martin Schnell, Shachi Mittal, Kianoush Falahkheirkhah, Anirudh Mittal, Kevin Yeh, Seth Kenkel, Andre Kajdacsy-Balla, P. Scott Carney, Rohit Bhargava. All-digital histopathology by infrared-optical hybrid microscopy. Proceedings of the National Academy of Sciences, 2020; 201912400 DOI: 10.1073/pnas.1912400117

Original Submission