| Title | E. Coli Created Using Synthetic DNA | |
| Date | Tuesday May 28 2019, @01:00PM | |
| Author | martyb | |
| Topic | ||
| from the debugging dept. | ||
Artificial life form given 'synthetic DNA'
UK scientists have created an artificial version of the stomach bug E. coli that is based on an entirely synthetic form of DNA. At the same time, Syn61 as they are calling it, has had its genetic code significantly redesigned. It's been done in a manner that will pave the way for designer bacteria that could manufacture new catalysts, drugs, proteins and materials.
[...] Syn61's 4 million genetic letters make this the largest entire genome to be synthesised from scratch. They were ordered in short segments from a laboratory supplies company, before being assembled into half-million-letter lengths in yeast cells by natural cellular machinery.
At this point, the genome engineers' job became a bit like a railway engineer's maintenance programme - replacing the E. coli genome piecewise - section by section - rather than all at once. "The bacterial chromosome is so big," team leader Jason Chin told the BBC, "we needed an approach that would let us see what had gone wrong if there had been any mistakes along the way." So it was only after each half-million-letter segment had been tested in partially synthetic bacteria that the eight segments were brought together in Syn61.
The approach is more cautious than that used by bio-entrepreneur, Craig Venter, whose microbial replicant based on the tiny organism Mycoplasma genitalium was presented to the world in 2010.
Also at PLOS, NYT, and Smithsonian.
Total synthesis of Escherichia coli with a recoded genome (DOI: 10.1038/s41586-019-1192-5) (DX)
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